Live cell imaging of GPCR trafficking and signaling in the endocytic compartment

A PhD fellowship is vacant as of September 1st, 2010 at the Bio-Imaging Center/Rudolf Virchow Center, University of Würzburg. The title of the project is: “Live cell imaging of GPCR trafficking and signaling in the endocytic compartment”.

G-protein-coupled receptors (GPCR) are a large family of plasma membrane receptors that mediate the action of neurotransmitters, hormones, ions, odorants, and light. Upon persistent stimulation, GPCR are transferred inside cells, an event that is generally thought to lead to signal termination. Very recently, we have discovered a new modality of GPCR signaling, in which GPCRs may continue to signal also after internalization in endosomes (*). This new type of intracellular signaling may have important consequences on both physiology and disease.

We are looking for a highly motivated candidate to join our efforts to further characterize this new type of intracellular GPCR signaling and explore its impacts on both physiological and pathological processes. Applicants should preferably have a background in cellular biology, biochemistry, pharmacology or endocrinology and a strong interest in live cell imaging and signal transduction. The project will require the use of 3D cell cultures and advanced microscopy techniques such as FRET and TIRF. Applications are invited from all nationalities, with a Master's degree (or Diploma) in a relevant area. The candidate is supposed to participate in the Graduate School of Life Sciences of the University of Würzburg (http://www.graduateschools.uni-wuerzburg.de/life_sciences/).

* Calebiro D, Nikolaev VO, Gagliani MC, de Filippis T, Dees C, et al. (2009) Persistent cAMP-Signals Triggered by Internalized G-Protein–Coupled Receptors. PLoS Biol 7(8): e1000172.

For further information please contact:

Davide Calebiro, M.D.
University of Würzburg
Bio-Imaging Center
Versbacher Strasse 9
97078 - Würzburg
e-mail: davide.calebiro@toxi.uni-wuerzburg.de
phone: +49 931 201 48710