Epigenetik

Task:
Aim of the research project is the characterization and analysis of the molecular mechanisms by which environmental events (stress, nutrition, drugs, etc.) lead to the formation of persistent cellular memory based on epigenetic changes in gene expression. A major experimental paradigm in mice will be on the enduring sex-specific alterations of the stress response through early-life experiences and how they couple to changes in DNA methylation of regulatory sequences of neuropeptide and nuclear receptor genes.

Techniques and methods:
Analysis of DNA methylation and chromatin marks (DNA extraction from micro dissections, bisulfite conversion, MSP-PCR, ultra deep sequencing, ChIP-seq), in vitro and in vivo ChIP, reporter assays, hormone and behavioral tests, primary neuronal cultures, generation of polyclonal antibodies, ISH/ICH.

Desired experiences and training:
Biochemistry, molecular genetics, molecular and cellular biology, protein analytic, experiences with genome-wide screens.

The project is part of a Marie Curie network (NINA). We are looking for individuals who are team-players, have organizational skills and who are interested in a multidisciplinary and international research project.

Only individuals who have NOT resided in Germany within the last 3 years are eligible for this position.